anti ha biotin antibody Search Results


anti-h7n9 hemagglutinin / ha antibody (biotin), hemagglutination inhibition activity, rabbit mab  (Sino Biological)
N/A
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anti-influenza a h3n2 (a-brisbane-10-2007) hemagglutinin-ha antibody-biotin  (TargetMol)
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biotin-conjugated rabbit anti-ha antibodies  (Immunology Consultants Laboratory Inc)
90
Immunology Consultants Laboratory Inc biotin-conjugated rabbit anti-ha antibodies
The lower section of the diagram shows the molecules bound to the surface of the streptavidin-coated beads at each step. Blue rectangles represent template DNA, green split arrows represent biotinylated <t>anti-HA</t> antibodies, turquoise clouds represent 3xHA-tagged hydrogenase proteins, blue and magenta circles represent C 12 -resazurin and C 12 -resorufin. A. DNA-bound beads are incubated with biotinylated anti-HA antibodies, added to a cell-free protein synthesis mixture, and emulsified into an oil phase. Emulsion CFPS transcribes mRNA from the bound DNA templates and synthesizes HA-tagged hydrogenase proteins, which are bound by the antibodies. The beads are then recovered from the emulsion, washed, and exposed to oxygen to challenge the hydrogenase mutants. The beads are then re-emulsified, and C 12 -resazurin is delivered to the emulsion droplets. Mutant hydrogenases that survive oxygen exposure consume hydrogen and reduce C 12 -resazurin to fluorescent C 12 -resorufin, which adsorbs to the bead surface. The beads are recovered and sorted by FACS. Fluorescent beads are added to a PCR mixture and thermocycled to recover DNA encoding improved hydrogenase mutants. B. An emulsion PCR step amplifies unique mutant templates to amounts sufficient to result in strong fluorescent signals. Beads are first incubated with biotinylated primers (represented by short black lines) and less than one molecule of template DNA per bead, then added to a PCR mixture and emulsified. The emulsion is thermocycled, and the beads are recovered, washed, and screened with the procedure shown in A .
Biotin Conjugated Rabbit Anti Ha Antibodies, supplied by Immunology Consultants Laboratory Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ha+biotin+antibody/biotin+conjugated+rabbit+anti+ha+antibodies/pmc02997796-127-8-14
Average 90 stars, based on 1 article reviews
biotin-conjugated rabbit anti-ha antibodies - by Bioz Stars, 2026-09
90/100 stars
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rat 3f10-pod conjugated antibody  (Merck KGaA)
90
Merck KGaA rat 3f10-pod conjugated antibody
The lower section of the diagram shows the molecules bound to the surface of the streptavidin-coated beads at each step. Blue rectangles represent template DNA, green split arrows represent biotinylated <t>anti-HA</t> antibodies, turquoise clouds represent 3xHA-tagged hydrogenase proteins, blue and magenta circles represent C 12 -resazurin and C 12 -resorufin. A. DNA-bound beads are incubated with biotinylated anti-HA antibodies, added to a cell-free protein synthesis mixture, and emulsified into an oil phase. Emulsion CFPS transcribes mRNA from the bound DNA templates and synthesizes HA-tagged hydrogenase proteins, which are bound by the antibodies. The beads are then recovered from the emulsion, washed, and exposed to oxygen to challenge the hydrogenase mutants. The beads are then re-emulsified, and C 12 -resazurin is delivered to the emulsion droplets. Mutant hydrogenases that survive oxygen exposure consume hydrogen and reduce C 12 -resazurin to fluorescent C 12 -resorufin, which adsorbs to the bead surface. The beads are recovered and sorted by FACS. Fluorescent beads are added to a PCR mixture and thermocycled to recover DNA encoding improved hydrogenase mutants. B. An emulsion PCR step amplifies unique mutant templates to amounts sufficient to result in strong fluorescent signals. Beads are first incubated with biotinylated primers (represented by short black lines) and less than one molecule of template DNA per bead, then added to a PCR mixture and emulsified. The emulsion is thermocycled, and the beads are recovered, washed, and screened with the procedure shown in A .
Rat 3f10 Pod Conjugated Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ha+biotin+antibody/rat+anti+ha+biotin+antibody+3f10/pm36671396-93-15-28
Average 90 stars, based on 1 article reviews
rat 3f10-pod conjugated antibody - by Bioz Stars, 2026-09
90/100 stars
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anti ha tag biotin antibody  (Abcepta)
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anti-ha tag antibody-biotin  (TargetMol)
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anti-influenza a h5n1 (a-anhui-1-2005) hemagglutinin-ha antibody-biotin  (TargetMol)
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anti-h7n9 hemagglutinin-ha antibody-biotin  (TargetMol)
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mouse anti-ha-tag antibody [+biotin]  (RayBiotech)
N/A
Mouse Anti-HA-Tag Antibody [+Biotin] Antibody, 50 µg
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Image Search Results


The lower section of the diagram shows the molecules bound to the surface of the streptavidin-coated beads at each step. Blue rectangles represent template DNA, green split arrows represent biotinylated anti-HA antibodies, turquoise clouds represent 3xHA-tagged hydrogenase proteins, blue and magenta circles represent C 12 -resazurin and C 12 -resorufin. A. DNA-bound beads are incubated with biotinylated anti-HA antibodies, added to a cell-free protein synthesis mixture, and emulsified into an oil phase. Emulsion CFPS transcribes mRNA from the bound DNA templates and synthesizes HA-tagged hydrogenase proteins, which are bound by the antibodies. The beads are then recovered from the emulsion, washed, and exposed to oxygen to challenge the hydrogenase mutants. The beads are then re-emulsified, and C 12 -resazurin is delivered to the emulsion droplets. Mutant hydrogenases that survive oxygen exposure consume hydrogen and reduce C 12 -resazurin to fluorescent C 12 -resorufin, which adsorbs to the bead surface. The beads are recovered and sorted by FACS. Fluorescent beads are added to a PCR mixture and thermocycled to recover DNA encoding improved hydrogenase mutants. B. An emulsion PCR step amplifies unique mutant templates to amounts sufficient to result in strong fluorescent signals. Beads are first incubated with biotinylated primers (represented by short black lines) and less than one molecule of template DNA per bead, then added to a PCR mixture and emulsified. The emulsion is thermocycled, and the beads are recovered, washed, and screened with the procedure shown in A .

Journal: PLoS ONE

Article Title: Development of an In Vitro Compartmentalization Screen for High-Throughput Directed Evolution of [FeFe] Hydrogenases

doi: 10.1371/journal.pone.0015275

Figure Lengend Snippet: The lower section of the diagram shows the molecules bound to the surface of the streptavidin-coated beads at each step. Blue rectangles represent template DNA, green split arrows represent biotinylated anti-HA antibodies, turquoise clouds represent 3xHA-tagged hydrogenase proteins, blue and magenta circles represent C 12 -resazurin and C 12 -resorufin. A. DNA-bound beads are incubated with biotinylated anti-HA antibodies, added to a cell-free protein synthesis mixture, and emulsified into an oil phase. Emulsion CFPS transcribes mRNA from the bound DNA templates and synthesizes HA-tagged hydrogenase proteins, which are bound by the antibodies. The beads are then recovered from the emulsion, washed, and exposed to oxygen to challenge the hydrogenase mutants. The beads are then re-emulsified, and C 12 -resazurin is delivered to the emulsion droplets. Mutant hydrogenases that survive oxygen exposure consume hydrogen and reduce C 12 -resazurin to fluorescent C 12 -resorufin, which adsorbs to the bead surface. The beads are recovered and sorted by FACS. Fluorescent beads are added to a PCR mixture and thermocycled to recover DNA encoding improved hydrogenase mutants. B. An emulsion PCR step amplifies unique mutant templates to amounts sufficient to result in strong fluorescent signals. Beads are first incubated with biotinylated primers (represented by short black lines) and less than one molecule of template DNA per bead, then added to a PCR mixture and emulsified. The emulsion is thermocycled, and the beads are recovered, washed, and screened with the procedure shown in A .

Article Snippet: Beads were then incubated in PBS with 200,000 biotin-conjugated rabbit anti-HA antibodies per bead (Immunology Consultants Laboratory, Inc., Newberg, OR) for two hours at room temperature.

Techniques: Incubation, Emulsion, Mutagenesis